EFFECT OF ACETYLSALICYLIC AND INDOLEBUTYRIC ACIDS ON in vitro ROOTING AND YIELD OF TOMATO (Lycopersicon esculentum Mill.).

Abstract

Adventitious shoots from in vitro leaf tissue of tomato (Lycopersicon esculentum Mill.) cv. Daniela were transferred to three culture media for rooting: a) the basic medium with Murashige and Skoog salts, 1 mg L^-1 thiamine, 100 mg L^-1 myo-inositol, 0.5 mg L^-1 pyridoxine, 0.5 mg L^-1 nicotinic acid, 30 g L^-1 sucrose, 5 g L^-1 agar, without growth regulators (MS0); b) medium MS0 with acetylsalicylic acid (MSAS); and c) medium MS0 with indolebutyric acid (MSIB). Shoots in media MSO, MSAS and MSIB developed roots in 4.2, 5.5 and 3.1 days respectively, and every shoot averaged 9.3, 8.6, and 14.3 adventitious roots. Plantlets from MSO and MSAS were transferred to pots and maintained in greenhouse for acclimatization. After 35 days, plants were transferred to 19 liters pots and cultured during 129 days for fruit production in an open hydroponic system. The plants obtained from seed, plants derived from medium MSAS and from medium MSO yielded 4.3, 3.8 and 3.4 kg fruit per plant. Seed plants and plants derived from medium MSAS accumulated more total biomass than those from medium MSO.