DETERMINATION OF GROWTH KINETIC AND STOICHOMETRIC PARAMETERS OF THE PLANT PATHOGEN Ralstonia solanacearum RsJ0314

Abstract

Ralstonia solanacearum is a phytopathogenic bacterium that causes bacterial wilt disease in economically important plants in several regions of the Americas. This study reports for the first time the kinetic characterization of strain RsJ0314 of R. solanacearum isolated in Mexico, which belongs to phylotype II. Three carbon sources that can be found in the xylem of the tomato plant were evaluated. The specific growth rate and doubling time in glucose were 1.9 and 1.8 times higher than in sucrose, while no growth was observed in maltose or fructose. This study contributes to the understanding of the kinetic parameters of growth of R. solanacearum, and provides information necessary for the development of biotechnological applications that assist in biological control of this bacterium. In addition, this study will serve as the basis for comparing growth with other strains of this species isolated in Mexico or other parts of the Americas.