EFFECT OF THE GLUCORAFANO ISOLATED FROM BROCCOLI FLORETS ON THE GERMINATION OF Colletotrichum gloeosporioides SPORES

Abstract

Anthracnose caused by Colletotrichum gloeosporioides, is the most important postharvest disease in mango (Mangifera indica L.) producing areas worldwide, and the strategy most used to control this disease is the pre-and post-harvest treatment with fungicides, but their use is increasingly restricted due to public awareness of hazardous residues in the fruits. Glucosinolates are natural products containing nitrogen and sulfur, mainly found in plants from the order Caparales, and their antimicrobial activity has been shown elsewhere. For this work we collected mango fruits with anthracnose symptoms, and from them the fungus Colletotrichum gloeosporioides was isolated and identified. Pathogen spores were placed on PDA containing different concentrations of glucoraphane (1.54, 0.92, 0.46, 0.15, 0.02 y 0 μg μL^-1) previously isolated from broccoli (Brassica oleracea cv. ‘Itálica’) florets. We evaluated spore germination until control treatment showed its highest percentage of germination. After 10 h of evaluation the concentrations of 1.54 and 0.92 μg μL^-1, caused complete inhibition of spore germination. The median lethal concentration was 0.65 μg μL^-1. Based on these results glucoraphane represents a friendly alternative for consumers, because of its capability to control anthracnose in harvested mango fruits.