GENETIC CHARACTERIZATION OF PINEAPPLE (Ananas spp.) ACCESSIONS BY RAPD AND ISSR

Abstract

Twenty nine ISSR primers (Inter Simple Sequence Repeat) and 20 decamer RAPD primers (Random Amplified Polymorphic DNA) were evaluated in 20 accesions of Ananas comosus, one of A. ananacoming and one of A. bracteatus, coming from the Germplasm Bank of the Campo Experimental del Papaloapan, Veracruz. The main objectives were to determine polymorphism within accesions, to characterize them and to detect duplicity of accesions in the bank. Only 20 ISSR and 12 RAPD primers were capable of detecting polymorphism, and the percent of polymorphism from ISSR was higer than in RAPD, (75.4 and 63.4, respectively). There was not duplicity within the accesions. Clusters generated by ISSR and RAPD were similar. All members of A. comosus were grouped close cluster while the wild accesions such as A. ananasoides and A. bracteatus, were grouped in the last clusters. Variations can be due to self incompatibility species, environmental factors, mutation rate and somaclonal variation. The were some differences in clusters generated by morphological data, mainly within A. comosus accessions.